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mcsf growth factor  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mcsf growth factor
    Mcsf Growth Factor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcsf+growth+factor/mcsf+1/pmc07113268-273-4-7
    Average 90 stars, based on 1 article reviews
    mcsf growth factor - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Regulatory T cells in central nervous system injury: A double-edged sword
    Article Snippet: Bone marrow was isolated from WT mice and cultured on untreated petri dishes in DMEM/F12 containing 10 ng/mL MCSF (eBioscience), 10% FCS, L-glutamine, and pen-strep (Invitrogen).

    Article Title: The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk.
    Article Snippet: Accepted Manuscript The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk Ilana Talior-Volodarky, Redouan Mahou, David Zhang, Michael Sefton PII: S0142-9612(17)30529-X DOI: 10.1016/j.biomaterials.2017.08.019 Reference: JBMT 18220 To appear in: Biomaterials Received Date: 30 May 2017 Revised Date: 4 August 2017 Accepted Date: 14 August 2017 Please cite this article as: Talior-Volodarky I, Mahou R, Zhang D, Sefton M, The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk, Biomaterials (2017), doi: 10.1016/j.biomaterials.2017.08.019.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Regulatory T cells in central nervous system injury: A double-edged sword
    Article Snippet: Macrophage skewing assay Bone marrow was isolated from WT mice and cultured on untreated petri dishes in DMEM/F12 containing 10 ng/mL MCSF (eBioscience), 10% FCS, L-glutamine, and pen-strep (Invitrogen).

    Article Title: Expression of NF-κB p50 in tumor stroma limits the control of tumors by radiation therapy.
    Article Snippet: Preparation of Bone Marrow Macrophages Bone marrow cells isolated from long-bones of mice were cultured for a total of 7 days in complete media containing 40 ng/ ml MCSF (Ebioscience), with additional growth media provided after 3 days of culture.

    Article Title: Endothelialization of arterial vascular grafts by circulating monocytes
    Article Snippet: VEGF (50 ng/ml) and MCSF (1 ng/ml; Thermo Fisher) were added to the EGM2 medium throughout the culture period.

    Modification:

    Article Title: Regulatory T cells in central nervous system injury: A double-edged sword
    Article Snippet: Bone marrow was isolated from WT mice and cultured on untreated petri dishes in DMEM/F12 containing 10 ng/mL MCSF (eBioscience), 10% FCS, L-glutamine, and pen-strep (Invitrogen).

    Article Title: The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk.
    Article Snippet: Accepted Manuscript The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk Ilana Talior-Volodarky, Redouan Mahou, David Zhang, Michael Sefton PII: S0142-9612(17)30529-X DOI: 10.1016/j.biomaterials.2017.08.019 Reference: JBMT 18220 To appear in: Biomaterials Received Date: 30 May 2017 Revised Date: 4 August 2017 Accepted Date: 14 August 2017 Please cite this article as: Talior-Volodarky I, Mahou R, Zhang D, Sefton M, The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk, Biomaterials (2017), doi: 10.1016/j.biomaterials.2017.08.019.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Regulatory T cells in central nervous system injury: A double-edged sword
    Article Snippet: Macrophage skewing assay Bone marrow was isolated from WT mice and cultured on untreated petri dishes in DMEM/F12 containing 10 ng/mL MCSF (eBioscience), 10% FCS, L-glutamine, and pen-strep (Invitrogen).

    Article Title: Expression of NF-κB p50 in tumor stroma limits the control of tumors by radiation therapy.
    Article Snippet: Preparation of Bone Marrow Macrophages Bone marrow cells isolated from long-bones of mice were cultured for a total of 7 days in complete media containing 40 ng/ ml MCSF (Ebioscience), with additional growth media provided after 3 days of culture.

    Article Title: Endothelialization of arterial vascular grafts by circulating monocytes
    Article Snippet: VEGF (50 ng/ml) and MCSF (1 ng/ml; Thermo Fisher) were added to the EGM2 medium throughout the culture period.

    Clinical Proteomics:

    Article Title: Regulatory T cells in central nervous system injury: A double-edged sword
    Article Snippet: Bone marrow was isolated from WT mice and cultured on untreated petri dishes in DMEM/F12 containing 10 ng/mL MCSF (eBioscience), 10% FCS, L-glutamine, and pen-strep (Invitrogen).

    Article Title: The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk.
    Article Snippet: Accepted Manuscript The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk Ilana Talior-Volodarky, Redouan Mahou, David Zhang, Michael Sefton PII: S0142-9612(17)30529-X DOI: 10.1016/j.biomaterials.2017.08.019 Reference: JBMT 18220 To appear in: Biomaterials Received Date: 30 May 2017 Revised Date: 4 August 2017 Accepted Date: 14 August 2017 Please cite this article as: Talior-Volodarky I, Mahou R, Zhang D, Sefton M, The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk, Biomaterials (2017), doi: 10.1016/j.biomaterials.2017.08.019.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Regulatory T cells in central nervous system injury: A double-edged sword
    Article Snippet: Macrophage skewing assay Bone marrow was isolated from WT mice and cultured on untreated petri dishes in DMEM/F12 containing 10 ng/mL MCSF (eBioscience), 10% FCS, L-glutamine, and pen-strep (Invitrogen).

    Article Title: Expression of NF-κB p50 in tumor stroma limits the control of tumors by radiation therapy.
    Article Snippet: Preparation of Bone Marrow Macrophages Bone marrow cells isolated from long-bones of mice were cultured for a total of 7 days in complete media containing 40 ng/ ml MCSF (Ebioscience), with additional growth media provided after 3 days of culture.

    Article Title: Endothelialization of arterial vascular grafts by circulating monocytes
    Article Snippet: VEGF (50 ng/ml) and MCSF (1 ng/ml; Thermo Fisher) were added to the EGM2 medium throughout the culture period.

    Isolation:

    Article Title: Regulatory T cells in central nervous system injury: A double-edged sword
    Article Snippet: Bone marrow was isolated from WT mice and cultured on untreated petri dishes in DMEM/F12 containing 10 ng/mL MCSF (eBioscience), 10% FCS, L-glutamine, and pen-strep (Invitrogen).

    Article Title: The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk.
    Article Snippet: Accepted Manuscript The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk Ilana Talior-Volodarky, Redouan Mahou, David Zhang, Michael Sefton PII: S0142-9612(17)30529-X DOI: 10.1016/j.biomaterials.2017.08.019 Reference: JBMT 18220 To appear in: Biomaterials Received Date: 30 May 2017 Revised Date: 4 August 2017 Accepted Date: 14 August 2017 Please cite this article as: Talior-Volodarky I, Mahou R, Zhang D, Sefton M, The role of insulin growth factor-1 on the vascular regenerative effect of MAA coated disks and macrophage-endothelial cell crosstalk, Biomaterials (2017), doi: 10.1016/j.biomaterials.2017.08.019.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Article Title: Regulatory T cells in central nervous system injury: A double-edged sword
    Article Snippet: Macrophage skewing assay Bone marrow was isolated from WT mice and cultured on untreated petri dishes in DMEM/F12 containing 10 ng/mL MCSF (eBioscience), 10% FCS, L-glutamine, and pen-strep (Invitrogen).

    Article Title: Expression of NF-κB p50 in tumor stroma limits the control of tumors by radiation therapy.
    Article Snippet: Preparation of Bone Marrow Macrophages Bone marrow cells isolated from long-bones of mice were cultured for a total of 7 days in complete media containing 40 ng/ ml MCSF (Ebioscience), with additional growth media provided after 3 days of culture.

    Article Title: Endothelialization of arterial vascular grafts by circulating monocytes
    Article Snippet: VEGF (50 ng/ml) and MCSF (1 ng/ml; Thermo Fisher) were added to the EGM2 medium throughout the culture period.



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    Male and female bone marrow-derived macrophages were cultured with 25 ng/mL <t>MCSF</t> alone or 25 ng/mL MCSF and 100 <t>ng/mL</t> <t>RANKL</t> for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.
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    Male and female bone marrow-derived macrophages were cultured with 25 ng/mL <t>MCSF</t> alone or 25 ng/mL MCSF and 100 <t>ng/mL</t> <t>RANKL</t> for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.
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    Image Search Results


    Male and female bone marrow-derived macrophages were cultured with 25 ng/mL MCSF alone or 25 ng/mL MCSF and 100 ng/mL RANKL for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.

    Journal: PeerJ

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression

    doi: 10.7717/peerj.14814

    Figure Lengend Snippet: Male and female bone marrow-derived macrophages were cultured with 25 ng/mL MCSF alone or 25 ng/mL MCSF and 100 ng/mL RANKL for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.

    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Techniques: Derivative Assay, Cell Culture, Lysis, RNA Extraction, Staining

    (A) Volcano plots depicting genes meeting fold change and p -value thresholds in MCSF and MCSF+RANKL treated cells. Differentials with −log10 transformed adjusted p -values of six and greater are plotted at the same level. Full volcano plots are included in . (B) MCSF-specific, MCSF+RANKL-specific, and common pathways altered during osteoclastogenesis. Pathways are listed in .

    Journal: PeerJ

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression

    doi: 10.7717/peerj.14814

    Figure Lengend Snippet: (A) Volcano plots depicting genes meeting fold change and p -value thresholds in MCSF and MCSF+RANKL treated cells. Differentials with −log10 transformed adjusted p -values of six and greater are plotted at the same level. Full volcano plots are included in . (B) MCSF-specific, MCSF+RANKL-specific, and common pathways altered during osteoclastogenesis. Pathways are listed in .

    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Techniques: Transformation Assay